LIU Jing ge, WU Jie ge, XU Shi yong, CHEN Qing, ZHANG Jin bi
Journal of Jinling Institute of Technology. 2022, 38(1): 85-85.
Abstract (
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Using the method of bioinformatics, the core promoter and transcription binding site are predicted in the candidate promoter region with a length of 2 000 bp upstream of the 5′ end of the pig hypoxia inducible factor HIF 1α published in NCBI. The physicochemical properties, subcellular localization, transmembrane domain, signal peptide, advanced structure and evolutionary relationship of the protein encoded by HIF 1α are analyzed. The results show that there is a potential promoter region at the upstream of 5′ end of pigs HIF1α. There are CpG islands and multiple transcription factor binding sites. The protein encoded by HIF 1α is a hydrophilic protein with an isoelectric point of 5.11. It is composed of 824 amino acids, and the highest content is leucine. HIF 1α protein is mainly located in the nucleus, and there is no transmembrane domain, so it is a non transmembrane protein. There is no signal peptide in the HIF 1α protein, and its secondary structure is mainly α spiral and random curl. In addition, through the comparison of HIF 1α sequence homology, it is found that sheep has the closest homology with pig s HIF 1α gene and the original chicken has the furthest homology. In conclusion, the study provides a theoretical basis for the exploration of structure and function of pig s HIF 1α gene.